University of Minnesota
University of Minnesota
College of Biological Sciences
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Methods for Experiment 321 -

ahue321 - Aboveground Biomass

Collection and Weighing

Two 16cm by 1.5 meter strips were clipped from each plot. See diagram for exact location of clip strips. Samples were taken back to the lab. Sorted to species, dried, and weighed.

ahwe321 - Species Percent Cover

Percent Cover Measuerments

On August 30th 2018, October 2019, and Aug 11, 2020 we sampled plant cover in all bison plots using a 1x1m quadrat in the center of each bison plot. We identified all plants rooted in the quadrat to species and assigned them a percent cover. We also assigned cover values to any bare ground or litter in the plot. Cover of plants can sum to over 100 if species overlap. Unknown species were IDed to closest taxonomic group possible.

ahye321 - Soil Carbon and Nitrogen

Collection and analysis

In the spring of each year soil cores were taken at two depths at 5 locations in each plot. All soil cores from the same depth were aggregated at the plot level. Soils were dried, ground, and sent to the lab for analysis of total carbon and nitrogen.

ahze321 - Oak sapling growth

Biomass Sorting Protoco

Pick up a tray with biomass from the pile. Make sure to get all trays for the plot if there is more than one tray. Sorted the plot to species for all species planted in the plot. Species that aren???t planted in the plot should be sorted into ???32 species weeds??? and ???real weeds??? which are any species other than the 32 species originally planted in the BigBio. Include a category for miscellaneous litter and mosses/lichens if necessary. Miscellaneous litter is categorized as anything that has not grown during the current growing season. Helpful tips for identifying miscellaneous litter: Any green plant part and any senesced plant part connected to a green plant part are considered this year???s growth. Anemone cylindrica and Lupinus perennis: All biomass that is not extremely decomposed (i.e. total lack of structural integrity, black color) is considered this year???s growth. Achillea millefolium: Any recognizable leaves (including black ones) are considered this year???s growth. Poa pratensis, Agropyron repens, Koeleria cristata, Bromus inermis, and Bouteloua gracilis: Anything that is light brown is considered this year???s biomass. Anything that is darker brown is considered to be litter. Andge, Schsc, and Sornu: Anything that is completely brown is considered litter. Anything that has any green on it or is connected to green biomass is considered this year???s growth. Solri, Petvi, Amoca, Lesca, and Asctu: Light brown biomass is considered this year???s growth. Dark brown material is classified as litter. If you cannot classify something by its color, anything that is recognizable as a certain species is classified as this year???s growth. Anything that is unrecognizable is litter. Make a separate bag for each sorted category in the plot. Label the bags with the Experiment Number Plot Number Species name Date sorted and put the corresponding biomass in each bag. Label a large plot bag with the Experiment Number, Plot Number, and Date sorted and place all bags of biomass into the plot bag. Check off the plot on the check off sheet. Place the bags into the drying room at the end of the day and check off all bags that go into the drying room.