University of Minnesota
University of Minnesota
College of Biological Sciences

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Methods for Experiment 169 -

Sampling Methods

We used a combined belowground and aboveground sampling of fungal structures. Sampling ectomycorrhizal roots belowground, with a combined morphotyping and RFLP approach, let us detect species such as hypogeous ascomycetes that were not detectable aboveground. Including aboveground sporocarp collections let us build a species list based on robust identifications with sporocarp vouchers, and permitted a much greater total area to be sampled. We sampled ectomycorrhizal fungi from six sites, three of which have had frequent controlled burns, and three of which have had no fire history during that period. See publication: Dickie, I.A., B.T.M. Dentinger, P.G. Avis, D.J. McLaughlin, P.B. Reich. 2009. Ectomycorrhizal fungal communities of oak savanna are distinct from forest communities. Mycologia 101(4):473?483. DOI: 10.3852/08-178