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Methods for Experiment 115 -

Materials and methods

Seeds of S. scoparium were added to 28 plots, each 2 by 2 meters in a newly abandoned agricultural field in June of 1993. Treatments were randomized among plots, which were arranged in two rows of 14. The field had been planted with annual rye since 1973 and farmed since 1896, and was disked prior to the seeding of S. scoparium. The seed-head material was purchasedfrom Prairie Restorations Inc. (Princeton, MN, USA), and contained about 180 live pure seeds per gram. Seed was added by weight, with eight treatment levels: a control with no seed added and seven levels of seed addition containing approximately 15, 45, 135, 405, 1,215, 3,645, and 10,935 live seeds per m2 . There were three replicates of each seed-addition level, and seven replicates of the control. Once seed was added, no further treatments were imposed.

Treatments

A. Control B. Addition of 0.333g of Schizacharium soparium C. Addition of 1.0g of Schizacharium soparium D. Addition of 3.0g of Schizacharium soparium E. Addition of 9.0g of Schizacharium soparium F. Addition of 27.0g of Schizacharium soparium G. Addition of 81.0g of Schizacharium soparium H. Addition of 729.0g of Schizacharium soparium 2m x 2m plots with 0.5m walkways located in North end of Lawrence plots strip. Area newly disked (from rye) on June 6, 1993. Three replicates per treatment, 8 treatments = 24 plots plus 3 extra controls = A Plot Number Treatment 1 F 2 D 3 E 4 C 5 A 6 D 7 B 8 A 9 E 10 C 11 H 12 F 13 D 14 H 15 G 16 C 17 A 18 F 19 A 20 A 21 E 22 G 23 B 24 A 25 H 26 G 27 B

pce115 - Plant species percent cover data

Percent Cover

Percent cover of all plant species in each plot was estimated visually.