University of Minnesota
University of Minnesota
College of Biological Sciences
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Methods for Experiment 071 -

Animal Sampling Methods

Pocket gophers are trapped out of E071 as soon as they appear (very seldom).

Field Operations: Cages

The 60cm x 60cm plots are enclosed by a cage of windowscreen which is 1.2m high.

Field Operations: Fencing

This experiment is within the fenced area of experiment E026 which is fenced to a height of 2m and to a depth of 1.3 m belowground to exclude all mammalian herbivores.

Field Operations: Mammal Trapping

Small mammals are trapped in the fall to discourage overwinter inhabitants. Pocket gophers are trapped out of the experiment as soon as they appear (very infrequent).

Field Operations: Watering

All plots are watered to receive at least 1.5cm/wk in April, May and October, and 2.5cm/wk in June through September.

Field Operations: Weeding

All plots and aisles are routinely weeded to keep the area free of unwanted plants and future seed sources.

Sampling

All grasshoppers in all cages are counted every 5 days during the experiment to determine the grasshopper "equilibrium" density. After the grasshoppers reached "equilibrium", vegetation was sampled by clipping a 50cm x 5cm strip. Soil samples were taken 5 times during the summer to determine the effect of grasshoppers on available soil nitrogen. In 1991, vegetation was sampled and sorted to species on June 24, 25 and 26. The clip strips were 60cm x 5cm. Vegetation was again sampled and sorted to species on September 3-5.

Treatment layout : trmte71

Additional Notes: Time Treatment: E=Early season=experiment done from last week of May to July 4 L=Late season=experiment done from last week of July to first week of September Vegetation Treatment: 1=SS 2=PP 3=LC 4=SS + PP 5=SS + LC 6=PP + LC where: SS=Schizachyrium scoparium, PP=Poa pratensis, LC=Lespedeza capitata Grasshopper Treatment: 1=none 2=MF 3=SC 4=MF + SC 5=PA 6=AC 7=AC + PA where MF=Pardalophora apiculata, SC=Arphia conspersa, PA=Melanoplus femur-rubrum,AC=Spharagemon collare

Field Identification Experiment Number Plot Number Vegetation Treatment Time Grasshopper Treatment
E7112L2
E7121L2
E7131L1
E7142L3
E7151L4
E7164L1
E7174L3
E7183L1
E7196L4
E71103L3
E71111L2
E71121E5
E71133E6
E71144E6
E71155E5
E71162E5
E71175L2
E71183L2
E71196E5
E71202L2
E71216L2
E71226E7
E71232L3
E71241E7
E71251E1
E71265L4
E71272E1
E7128-2-2-2
E71292L4
E71303E1
E71311E6
E71323E7
E71334L2
E71343L4
E71353L2
E71364L4
E71373E5
E71386E1
E71395L1
E71404L3
E71411L3
E71423L4
E71435E6
E71442E7
E71452L1
E71466L3
E71473L3
E71483L1
E71494E5
E71504L4
E71516L1
E71525E7
E71531L3
E71542L4
E71555E5
E71564E1
E71576E6
E71585E1
E71592E6
E71603L3
E71616L3
E71621L4
E71636E7
E71641L4
E71656L4
E71662L2
E71674L1
E71683L2
E71692L4
E71702L1
E71715E1
E71724L2
E71734E7
E71745E6
E71756E6
E71762E5
E71771E5
E71782E6
E71796L2
E71801E6
E71816L2
E71825L3
E71834L2
E71846E1
E71856E5
E71862E7
E71871E1
E71883E7
E71894L4
E71903L1
E71916E7
E71921E7
E71935E7
E71944L1
E71956L1
E71962L3
E71973E5
E71985L2
E71991L1
E711004E5
E711015L4
E711022E7
E711033L4
E711042E6
E711055E6
E711062E1
E711074E7
E711082L3
E711092E5
E711106E1
E711112E1
E711121L3
E711135L1
E711146L3
E711152E1
E711165E5
E711173L2
E711186E6
E711195E1
E711206L4
E711213E6
E711222L4
E711232L1
E711246E5
E711252L2
E711261E1
E711274E6
E711284L3
E711292L1
E711304L4
E711315L1
E711323L1
E711334E1
E711345L4
E711353E1
E711365E7
E711373E5
E711382E6
E711394E1
E711406E6
E711414E7
E711421L4
E711434E5
E711444L1
E711451L4
E711464L4
E711472L4
E711481E1
E711494L3
E711501L1
E711516L4
E711525E7
E711535E1
E711543L4
E711552L4
E711564E6
E711576E5
E711581E5
E711593E6
E711602E5
E711613E7
E711622E5
E711632L1
E711644L4
E711656L2
E711663E1
E711671E6
E711686E1
E711693E7
E711701E7
E711714E6
E711724L3
E711733E6
E711744E6
E711756E7
E711762E7
E711776E7
E711785L2
E711791L2
E711803E5
E711813L3
E711826E5
E711834E1
E711843L4
E711854L1
E711865L3
E711872L3
E711885E6
E711891E7
E711903E5
E711914E5
E711923L2
E711935L4
E711944E7
E711956L3
E711964L2
E711971E7
E711985L2
E711995E5
E712002E1
E712015L1
E71202???
E712034E7
E712045E6
E712053E7
E712064E1
E712071L3
E712081L2
E712093E1
E712106E6
E712111L1
E712126L2
E712134E5
E712142E1
E712153L1
E712165L3
E712176L3
E712181L4
E712191E6
E712205L1
E712216E1
E712221L3
E712231E6
E712243E1
E712255L4
E712262E6
E712273E6
E712281E5
E712293L3
E712301L1
E712315E7
E712321E5
E712335E1
E712345L3
E712356L1
E712365L2
E712376L4
E712385L3
E712395E5
E712401E1
 

Watering Methods

All plots receive at least 1.5cm/week of water in April, May and October and 2.5cm/week in June through September. If the plots receive this water from natural rainfall, then no supplemental watering is needed. When watering is needed, it is done using a system of hoses set up in the field. A black irrigation pipe, 3 inches in diameter, is hooked up to the pump. This extends out to the E026 area where the 3-inch hose is divided into two 2-inch hoses. One part goes toward the E026 (and E071) plots (the other toward E055, E069, E070). From this hose, several regular garden hoses are connected. Gardena Excellent Oscillating Sprinklers, model 6968, are attached to these hoses. These sprinklers are purchased at Sears and made at Gardena, Inc., 6031 Culligan Way, Minnetonka, MN 55345. Three or four hoses/sprinklers are used at one time. Any more than this reduces the water pressure enough so the sprinklers will not work. The sprinklers are rotated in order to reach all of the plots.