FAB 1 : Forests and Biodiversity Experiment - High density diversity experiment - Experiment 271

SSU amplicons of arbuscular mycorrhizal fungal communities in soils

Years Available: 2016

Principle Investigators

Kennedy, Peter

SSU amplicons of arbuscular mycorrhizal fungal communities in soils

Arbuscular mycorrhizal fungal communities in soils of FAB experiment at Cedar Creek Ecosystem Science Reserve, Minnesota, USA While the relationship between plant and microbial diversity has been well studied in grasslands, less is known about similar relationships in forests, especially for obligately symbiotic arbuscular mycorrhizal (AM) fungi. To assess the effect of varying tree diversity on microbial alpha- and beta-diversity, we sampled soil from plots in a high-density tree diversity experiment in Minnesota, USA three years after establishment. Three of 12 tree species are AM hosts; the other 9 primarily associate with ectomycorrhizal fungi. We used phospho- and neutral lipid fatty acid analysis to characterize the biomass and functional identity of the whole soil bacterial and fungal community and high throughput sequencing to identify the species-level richness and composition of the AM fungal community. Results are published in: Grossman, J. J., Butterfield, A. J., Cavender-Bares, J., Hobbie, S. E., Reich, P. B., Gutknecht, J., & Kennedy, P. G. (2019). Non-symbiotic soil microbes are more strongly influenced by altered tree biodiversity than arbuscular mycorrhizal fungi during initial forest establishment. FEMS microbiology ecology, 95(10). doi:10.1093/femsec/fiz134

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