Citation. Dijkstra, F.A.; West, J.B.; Hobbie, S.E.; Reich, P.B.; Trost, J. 2007. Plant diversity, CO₂, and N influence inorganic and organic n leaching in grasslands. ECOLOGY 88:490-500.

Abstract. In nitrogen (N)-limited systems, the potential to sequester carbon depends on the balance between N inputs and losses as well as on how efficiently N is used, yet little is known about responses of these processes to changes in plant species richness, atmospheric CO₂ concentration ([CO₂]), and N deposition. We examined how plant species richness (1 or 16 species), elevated [CO₂] (ambient or 560 ppm), and inorganic N addition (0 or 4 g·m⁻²·yr⁻¹) affected ecosystem N losses, specifically leaching of dissolved inorganic N (DIN) and organic N (DON) in a grassland field experiment in Minnesota, USA. We observed greater DIN leaching below 60 cm soil depth in the monoculture plots (on average 1.8 and 3.1 g N·m⁻²·yr⁻¹ for ambient N and N-fertilized plots respectively) than in the 16-species plots (0.2 g N·m⁻²·yr⁻¹ for both ambient N and N-fertilized plots), particularly when inorganic N was added. Most likely, loss of complementary resource use and reduced biological N demand in the monoculture plots caused the increase in DIN leaching relative to the high-diversity plots. Elevated [CO₂] reduced DIN concentrations under conditions when DIN concentrations were high (i.e., in N-fertilized and monoculture plots). Contrary to the results for DIN, DON leaching was greater in the 16-species plots than in the monoculture plots (on average 0.4 g N·m⁻²·yr⁻¹ in 16-species
plots and 0.2 g N·m⁻²·yr⁻¹ in monoculture plots). In fact, DON dominated N leaching in the 16-species plots (64% of total N leaching as DON), suggesting that, even with high biological demand for N, substantial amounts of N can be lost as DON. We found no significant main effects of elevated [CO₂] on DIN or DON leaching; however, elevated [CO₂] reduced the positive effect of inorganic N addition on DON leaching, especially during the second year of observation. Our results suggest that plant species richness, elevated [CO₂], and N deposition alter DIN loss primarily through changes in biological N demand. DON losses can be as large as DIN loss but are more sensitive to organic matter production and turnover.

Key Words. dissolved inorganic nitrogen, dissolved organic nitrogen, drainage, elevated CO₂, grassland, leaching, lysimeter, nitrogen deposition, nitrogen loss, plant species
richness