Field operations for E110

Draining of rain water

A small trench is maintained to the south side of drought plots to let sheltered-off water drain away. The dirt from these trenches is stored for later replacement on a sheet of plastic adjacent to the site in each field. Precipitation measurement: Modified 1.5 l plastic Coke bottles are used (2 per treatment in each field) to measure precipitation in the drought season (day 130 through day 240). Outside this period data from the weather station at Cedar Creek are used. Mammal trapping: Gophers are trapped and removed from field A as they appear, in field C they are not a problem.

Seedbank

Seedbank samples (2"wide, depths 0-1.5cm, 1.5-5.75cm, 5.75-10cm) are cut on October 24, two per plot, hammering an alu tube down. Samples are stored over the winter in a dark fridge (4 C) and germinated in the spring in first week of April.

Soil nitrogen

Nitrogen is sampled (0.01M KCl) mid monthly from April through November. To assess mineralization PVC 1", 1 ft tubes are inserted 5 cm away at the time of sampling to prevent uptake and leaching, and collected the next month.

Germination

Germination is monitored in 0.1x1m strips in each plot every two weeks, to begin in early May. Plants are staked and positioned with coordinates.

Plant demography

Approximately 20 individuals per plot per species are staked and positioned with coordinates, randomly chosen across the entire 3x3 m plot area. Plots are monitored three times per season: at the start of the early drought (middle May); at the end of early and the start of late drought (beginning of July); and at the end of the late drought (end of August).

Below ground biomass

Rootcores are taken with 2" alu pipe, 0-10; 10-20 etc to 100cm depth in each plot in end October. Samples are weighed, air dried, weighed and sieved.

Above ground biomass

Clipping and sorting of a 10x300 cm strip is done in early August. A 'clipping frame' is used to enclose and demark the vegetation to be clipped by descending it down vertically onto the plot, and clipping all vegetation enclosed (including parts that might cross the plot but grow outside).

Soil moisture

Moisture profiles to 1m depth are measured through a neutron probe (and gravimetrically for shallowest samples) every two weeks.


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