Statistical analysis
Two-way ANOVA for unbalanced designs was used to evaluate the effects of host species and soil mixture on total spore densities, species richness and species diversity of the VAM fungal communities. Relative spore densities were arcsine-square root transformed (Zar, 1984) and then two-way ANOVA was used to evaluate the effects of host species and soil mixture on the densities of 12 VAM fungal species which occurred in 25% or more of the plots. Tukey's test was used to make multiple comparisons of means. Spearman rank correlation analysis was conducted to examine the relationships between spore densities of individual VAM fungal species and total soil C (which was highly correlated with both total N and % organic matter) and successional ranking. Agrostis, Agropyron, Poa, Schizachyrium, and Andropogon were assigned successional rankings of 1 through 5 respectively based on their relative abundance in the successional grasslands at CCNHA (Tilman, 1988). ANOVA and correlation analyses were performed using STATGRAPHICS (STSC, 1986).
Plot: Plot number
Host:
Fertil: Ranking of fertilizer
Ntot: Initial total N
pAmor: % A morrow
pAscr: % A. scrobiculater
pAfov: % A foresto
pEinf: % E. infrequens
| Plot | Host | Fertil | Ntot(1986) | pAmor | pAscr | pAf ov | pEinf |
|---|
| 1 | 1 | 3 | 890.61 | 0 | 0 | 0 | 0 |
| 1 | 2 | 3 | 914.03 | 0 | 0 | 0 | 0.741370 |
| 1 | 3 | 3 | 697.86 | 0 | 0 | 0 | 0 |
| 1 | 5 | 3 | 872.15 | 0 | 0 | 0 | 0 |
| 1 | 7 | 3 | 843.15 | 0 | 0 | 0 | 0 |
| 2 | 1 | 1 | 231.89 | 0 | 0 | 0 | 0 |
| 2 | 2 | 1 | 241.57 | 0 | 0 | 0 | 0 |
| 2 | 3 | 1 | 278.33 | 0 | 0 | 0 | 0 |
| 2 | 5 | 1 | 246.82 | 0 | 0 | 0 | 0 |
| 2 | 7 | 1 | 244.65 | 0 | 0 | 0 | 0 |
| 3 | 1 | 1 | 79.40 | 0 | 0 | 0 | 0 |
| 3 | 2 | 1 | 96.77 | 0 | 0 | 0 | 0 |
| 3 | 3 | 1 | 97.60 | 0.155096 | 1.395870 | 0 | 0.155096 |
| 3 | 5 | 1 | 78.62 | 0 | 0 | 0 | 0 |
| 3 | 7 | 1 | 81.27 | 0 | 0.345225 | 0 | 0 |
| 4 | 1 | 3 | 605.61 | 0 | 0 | 0 | 0 |
| 4 | 2 | 3 | 447.44 | 0 | 0 | 0.368293 | 0 |
| 4 | 3 | 3 | 534.71 | 0 | 0 | 0 | 0.132997 |
| 4 | 5 | 3 | 689.80 | 0 | 0 | 0 | 0 |
| 4 | 7 | 3 | 555.22 | 0 | 0 | 0 | 0.922248 |
| 5 | 1 | 4 | 1523.00 | 0 | 0 | 0 | 0.656255 |
| 5 | 2 | 4 | 1686.00 | 0 | 0 | 0 | 3.211392 |
| 5 | 3 | 4 | 1788.00 | 0 | 0 | 0 | 0.588464 |
| 5 | 5 | 4 | 1575.00 | 0 | 0 | 0 | 0 |
| 5 | 7 | 4 | 1609.00 | 0.163508 | 0.163508 | 0 | 0.490524 |
| 6 | 1 | 1 | 226.78 | 0 | 0 | 0 | 0 |
| 6 | 2 | 1 | 239.91 | 0 | 0 | 0 | 0.180370 |
| 6 | 3 | 1 | 196.73 | 0.345029 | 0 | 0 | 0 |
| 6 | 5 | 1 | 198.26 | 0 | 0 | 0 | 0 |
| 6 | 7 | 1 | 222.74 | 0.520875 | 0 | 0 | 0.057875 |
| 7 1 3 1148.11 0 0 0 1.580219 |
| 7 2 3 1114.33 0.479236 0 0 0 |
| 7 3 3 1061.20 0 0 0 0 |
| 7 5 3 1163.92 0.391283 0 0 0 |
| 7 7 3 1076.06 0 0 0 0 |
| 8 1 2 551.41 0 0 0 0.488860 |
| 8 2 2 498.08 0 0 0 0.963350 |
| 8 3 2 484.56 0 0 0 0.094143 |
| 8 5 2 553.69 0 0 0 0 |
| 8 7 2 488.19 0 0.285923 0 0 |
| 9 1 2 375.56 0 0.894361 0 0.447180 |
| 9 2 2 424.55 0 0 0 0.247226 |
| 9 3 2 424.35 0 0 0 0 |
| 9 5 2 376.12 0 0.668618 0 0 |
| 9 7 2 410.85 0.170429 0 0 0 |
| 10 1 2 501.04 0 0.261424 0 0 |
| 10 2 2 447.06 0 1.136443 0 0 |
| 10 3 2 497.79 0.488798 0.081466 0 0.162932 |
| 10 5 2 489.95 0 0 0 0 |
| 10 7 2 509.62 0 0 0 0.233439 |
| 11 1 3 1100.00 0 0 0 0 |
| 11 2 3 1100.00 0 0 0 1.175336 |
| 11 3 3 1100.00 0 0 0 0.345439 |
| 11 5 3 1100.00 0 0 0 0 |
| 11 7 3 1100.00 0 0.192151 0 0.084648 |
Plot: Plot number
pGigi: % Gigaspora gigantea
pGisp: % G. sp
pGlag: % Glomes aggregatus
pGlet: % G. etoricatum
pGlfa: % G. fasiculatum
pGlge: % G. geosporum
| Plot | Host | pGigi | pGisp | pGlag | pGlet | pGlfa | pGlge |
|---|
| 1 | 1 | 0 | 0.082708 | 67.58229 | 0 | 0 | 0 |
| 1 | 2 | 0 | 0 | 56.71977 | 0.494246 | 0 | 0 |
| 1 | 3 | 0.111697 | 0.111697 | 38.68058 | 1.447936 | 0 | 0 |
| 1 | 5 | 0 | 0 | 47.71113 | 0.863550 | 0 | 0 |
| 1 | 7 | 0 | 0.123087 | 37.837.87 | 1.139694 | 0.227938 | 0 |
| 2 | 1 | 0.523183 | 0.784775 | 99.15950 | 0.645906 | 0 | 0 |
| 2 | 2 | 0 | 0 | 62.79936 | 2.015148 | 0.183195 | 0 |
| 2 | 3 | 0.300433 | 0.159679 | 73.33891 | 0 | 0.236561 | 0 |
| 2 | 5 | 0 | 0 | 49.05587 | 0 | 0 | 0 |
| 2 | 7 | 0 | 0.050210 | 29.59233 | 1.115779 | 0 | 0 |
| 3 | 1 | 0.362872 | 3.723073 | 95.39921 | 0 | 0 | 0 |
| 3 | 2 | 0 | 0.065172 | 76.84587 | 0.724141 | 0 | 0 |
| 3 | 3 | 0 | 1.733981 | 61.86344 | 0.310193 | 0 | 0 |
| 3 | 5 | 0 | 1.308479 | 24.48552 | 1.211554 | 0 | 0 |
| 3 | 7 | 0.418619 | 6.907225 | 45.62682 | 0.543661 | 0 | 0 |
| 4 | 1 | 0.127230 | 0.381691 | 50.80270 | 2.356122 | 0 | 0 |
| 4 | 2 | 0 | 0.696074 | 33.88299 | 0 | 0 | 0 |
| 4 | 3 | 0.035909 | 0.107727 | 41.40606 | 2.127957 | 0 | 0 |
| 4 | 5 | 0 | 0 | 54.49664 | 0.832613 | 0 | 0 |
| 4 | 7 | 0.186755 | 0 | 27.43689 | 0 | 0 | 0 |
| 5 | 1 | 0 | 0.354377 | 45.12192 | 1.093758 | 0 | 0 |
| 5 | 2 | 0 | 0 | 73.67737 | 3.612816 | 0 | 0 |
| 5 | 3 | 0 | 0.079442 | 57.37527 | 0.147116 | 0 | 0 |
| 5 | 5 | 0 | 0 | 43.97329 | 1.833043 | 5.084103 | 0 |
| 5 | 7 | 0.044147 | 0 | 30.41249 | 0.981048 | 0.327016 | 0.163508 |
| 6 | 1 | 0.114548 | 0.305462 | 78.28455 | 0 | 0 | 0 |
| 6 | 2 | 0.048700 | 0.282200 | 96.11779 | 0.901853 | 0 | 0 |
| 6 | 3 | 0.093157 | 0.784941 | 74.29341 | 0 | 0.086257 | 0 |
| 6 7 0.062505 0.078131 56.40562 0.057875 0 0.173625 |
| 7 1 0 0 33.31102 0 0 0 |
| 7 2 0 0 63.25682 0.479236 0 0 |
| 7 3 0.077668 0 33.15305 2.013634 0.086257 0 |
| 7 5 0 0 17.80339 0 52.84282 0 |
| 7 7 0 0 24.88392 0 0 0 |
| 8 1 0.244430 0.989943 69.52823 0.488860 0 0 |
| 8 2 0 0.780314 32.28187 0 0.963350 0 |
| 8 3 0.170399 0.559210 39.63804 2.165293 0 0 |
| 8 5 0 0.562054 53.12873 0 0.416336 0 |
| 8 7 0 2.547581 25.48438 1.715542 0 0 |
| 9 1 0.241477 0.482955 45.88076 0.894361 0 0 |
| 9 2 0 0.100126 50.80865 0.494452 0 0 |
| 9 3 0.042618 0.954971 22.62255 0.157846 0 0 |
| 9 5 0 0.722108 34.70132 0.334309 0 0 |
| 9 7 0.154238 0.230079 27.04456 0.170429 0 0 |
| 10 1 0 0.282338 67.50773 0.522849 0 0.070584 |
| 10 2 0 0.184103 51.78773 0 0 0 |
| 10 3 0 0.323421 44.80325 0.673727 0 0 |
| 10 5 0 0.073667 35.96356 0.545687 0 0 |
| 10 7 0.179748 0.378171 43.68585 0.116719 0 0 |
| 11 1 1.011293 0.060677 49.55337 3.708075 0 0 |
| 11 2 0 0 56.55559 2.888538 0 0 |
| 11 3 0 0.062179 16.58107 1.381756 0.345439 0 |
| 11 5 0.249035 0 13.83529 0 0 0 |
| 11 7 0 0 18.03005 0.592536 0.846481 0 |
pGlin: % G.intravadix
pGlle: % G.letotium
| Plot | Host | pGlin | pGlle | pGlmac | pGlmiag | pGlmo | pGloc |
|---|
| 1 | 1 | 0 | 25.01175 | 4.371314 | 3.369618 | 0 | 4.288605 |
| 1 | 2 | 0 | 42.73258 | 1.976987 | 3.706851 | 2.471234 | 1.482740 |
| 1 | 3 | 0 | 52.63248 | 3.102720 | 0 | 1.145938 | 14.89305 |
| 1 | 5 | 0 | 60.05126 | 1.079437 | 0 | 0 | 0 |
| 1 | 7 | 0 | 60.34684 | 2.963206 | 0 | 1.139694 | 5.470535 |
| 2 | 1 | 0 | 5.390086 | 0.645906 | 0 | 0 | 0 |
| 2 | 2 | 0 | 37.61733 | 0.366390 | 0 | 0.732781 | 3.480711 |
| 2 | 3 | 0 | 25.50846 | 2.129056 | 0 | 0 | 4.612956 |
| 2 | 5 | 0 | 60.67772 | 0 | 0 | 0 | 0 |
| 2 | 7 | 0 | 59.37620 | 0.371926 | 0 | 0 | 16.92265 |
| 3 | 1 | 0 | 3.991599 | 0.362872 | 0 | 0 | 0.725745 |
| 3 | 2 | 0.241380 | 22.70.907 | 1.689663 | 2.413804 | 0 | 0.965521 |
| 3 | 3 | 5.209700 | 14.11380 | 0.930580 | 0 | 0 | 20.47277 |
| 3 | 5 | 0.605777 | 63.40065 | 3.634664 | 8.480883 | 0 | 1.211554 |
| 3 | 7 | 0 | 32.06246 | 0 | 17.95443 | 0 | 0.543661 |
| 4 | 1 | 0 | 50.16655 | 0.942448 | 0 | 1.540903 | 0 |
| 4 | 2 | 0 | 49.80799 | 22.56533 | 0 | 1.841466 | 0 |
| 4 | 3 | 0.265994 | 62.09779 | 1.329973 | 3.052289 | 0 | 0 |
| 4 | 5 | 0 | 52.97296 | 1.248920 | 0 | 0 | 0 |
| 4 | 7 | 0 | 72.94411 | 0.345843 | 6.582550 | 0 | 1.152810 |
| 5 | 1 | 0 | 58.97766 | 1.531262 | 0 | 0.218751 | 1.093758 |
| 5 | 2 | 0 | 19.86246 | 2.408544 | 0 | 1.204272 | 6.021360 |
| 5 | 3 | 0 | 43.28449 | 2.059625 | 0 | 0.588464 | 7.502920 |
| 5 | 5 | 0 | 59.13831 | 0.329387 | 0.823469 | 0.627484 | 1.482245 |
| 5 | 7 | 0 | 45.18708 | 1.471572 | 0.327016 | 2.779636 | 28.61390 |
| 6 | 1 | 0 | 25.18647 | 0 | 2.545517 | 0.14147 | 0 |
| 6 | 2 | 0.901853 | 8.075200 | 0.180370 | 0 | 0 | 0 |
| 6 | 3 | 0 | 27.25730 | 0.172514 | 1.050613 | 0.258771 | 2.242689 |
| 6 | 5 | 0 | 33.74706 | 0.200178 | 0.200178 | 0 | 4.003566 |
| 6 | 7 | 0 | 45.37984 | 1.887884 | 0 | 0.231500 | 1.967752 |
| 7 | 1 | 0 | 46.06339 | 20.54284 | 0 | 1.003439 | 4.740657 |
| 7 | 2 | 0 | 25.72781 | 6.774008 | 0 | 0.783551 | 9.105494 |
| 7 3 0.287662 66.18529 6.040903 1.725972 1.438310 2.013634 |
| 7 5 0 22.60444 0.195641 19.56417 0.391283 0.195641 |
| 7 7 0 73.07664 0.769605 0 1.677740 8.978735 |
| 8 1 0 28.28059 0.488860 3.666456 0.733291 3.177595 |
| 8 2 0 66.39412 0.963350 0 1.575078 3.853402 |
| 8 3 0 59.76962 1.409323 0 1.111830 3.859870 |
| 8 5 0 46.79625 1.665347 5.620546 0.224821 0.416336 |
| 8 7 0.571847 63.77244 0 0.571847 1.738416 12.58064 |
| 9 1 0 50.39282 1.341542 0 0 2.683085 |
| 9 2 0 55.85948 0.247226 0 0.247226 0 |
| 9 3 0 82.67523 0.315692 0 0 2.841236 |
| 9 5 0 70.91706 0 0 0.605100 0.668618 |
| 9 7 0.852146 77.10815 0.170429 0.255643 0.347675 2.812082 |
| 10 1 0 36.88965 0.522849 5.076869 0.784274 0.261424 |
| 10 2 0 49.63758 0.454577 0 1.375096 2.954753 |
| 10 3 0.162932 56.01710 1.059063 0.733197 0.865173 3.503054 |
| 10 5 0 62.88235 1.091375 7.366787 0.619355 0 |
| 10 7 0.233439 61.72721 0 0 0.233439 1.050477 |
| 11 1 1 49.71517 7.079053 0 1.348391 0 |
| 11 2 0 25.57850 19.18387 0 3.884586 1.992095 |
| 11 3 0 79.90005 9.326854 0 0.939594 4.145268 |
| 11 5 0 95.87860 0 0 1.632564 0 |
| 11 7 0 73.25872 2.031555 12.15462 0.953984 2.708740 |
| Plot | Host | pScal | pSery | pSper | Totspore |
|---|
| 1 | 1 | 6.126578 | 0 | 0.744379 | 1349.037 |
| 1 | 2 | 0.494246 | 0 | 0.088964 | 1246.666 |
| 1 | 3 | 0.827392 | 0 | 0 | 2022.481 |
| 1 | 5 | 3.238312 | 0 | 0 | 2005.296 |
| 1 | 7 | 1.490720 | 0 | 0 | 1806.370 |
| 2 | 1 | 0 | 0 | 0.697578 | 1236.814 |
| 2 | 2 | 0 | 0 | 0 | 2167.185 |
| 2 | 3 | 0 | 0 | 0.373767 | 3339.814 |
| 2 5 0.530678 0 0.960527 776.2592 |
| 2 7 0 0 0.125525 4284.185 |
| 3 1 0.097975 0 0.254461 1072.259 |
| 3 2 0 0 0 1621.148 |
| 3 3 0 0.310193 0 2546.814 |
| 3 5 0.163559 0.163559 0.490679 642.9259 |
| 3 7 0.073394 0 1.027521 1437.481 |
| 4 1 3.081807 0 0.254461 861.8518 |
| 4 2 0.368293 0 0.099439 1102.481 |
| 4 3 0.531989 0 0.071818 3095.592 |
| 4 5 0.416306 0 0 1956.666 |
| 4 7 0 0 0.217881 3544.370 |
| 5 1 2.625021 0 0.059062 1891.740 |
| 5 2 0.401424 0 0 1018.592 |
| 5 3 1.912509 0 0 2858.370 |
| 5 5 0 0 0 2547.740 |
| 5 7 2.125604 0 0.220735 2570.296 |
| 6 1 0.424252 0 0 2802.370 |
| 6 2 0.180370 0 0 2194.629 |
| 6 3 0.109546 0 0.093157 4585.222 |
| 6 5 0.127113 0 0.054048 3905.851 |
| 6 7 0.073501 0 0 6840.851 |
| 7 1 5.530767 0 0.426659 530.6296 |
| 7 2 0.958473 0 0.064696 1700.629 |
| 7 3 0 0 0.155337 1456.074 |
| 7 5 0 0 0 2157.925 |
| 7 7 0.513070 0 0 1645.925 |
| 8 1 2.199873 0 0.244430 1674.814 |
| 8 2 0.963350 0 0 836.1111 |
| 8 3 0.213705 0 0.432117 4304.851 |
| 8 5 0.832673 0 0.449643 979.5555 |
| 8 7 0 0 0.231598 1418.407 |
| 9 1 4.918990 0 0.845172 902.9629 |
| 9 2 0.370839 0 0 3247.148 |
| 9 3 0.157846 0 0.383566 2584.592 |
| 9 5 0.334309 0 0 1207.037 |
| 9 7 0.255643 0 0.092031 4766.333 |
| 10 1 1.045699 0 0.705847 1614.111 |
| 10 2 0 0 0.061367 1753.222 |
| 10 3 0 0 0.219959 4959.740 |
| 10 5 0.545687 0 0.272843 1484.518 |
| 10 7 0 0 0.283628 3430.888 |
| 11 1 0 0 0.121355 1855.666 |
| 11 2 0.358577 0 0 622.5555 |
| 11 3 0.345439 0 0.062179 1824.333 |
| 11 5 1.383529 0 0 453.6666 |
| 11 7 0.169296 0 0.068564 4860.703 |
richness: number of VAM fungal species
Nostoc: Bluegreen alfal colonies
| Plot | Host | Richness | Nostoc | Date |
|---|
| 1 | 1 | 8 | 2 | 07-16-90 |
| 1 | 2 | 10 | 0 | 07-07-90 |
| 1 | 3 | 9 | 0 | 08-08-90 |
| 1 | 5 | 7 | 8 | 08-10-90 |
| 1 | 7 | 10 | 0 | 08-16-90 |
| 2 | 1 | 7 | 23 | 07-05-90 |
| 2 | 2 | 7 | 190 | 08-02-90 |
| 2 | 3 | 8 | 7 | 08-02-90 |
| 2 | 5 | 4 | 190 | 08-02-90 |
| 2 | 7 | 7 | 20 | 08-03-90 |
| 3 | 1 | 8 | 10 | 08-17-90 |
| 3 | 2 | 8 | 0 | 08-17-90 |
| 3 | 3 | 11 | -- | 08-17-90 |
| 3 | 5 | 11 | 7 | 08-19-90 |
| 3 | 7 | 10 | 5 | 08-20-90 |
| 4 1 9 181 08-20-90 |
| 4 2 8 -- 11-15-89 |
| 4 3 11 -- 08-20-90 |
| 4 5 5 16 08-21-90 |
| 4 7 9 8 08-21-90 |
| 5 1 10 0 08-06-90 |
| 5 2 8 0 08-06-90 |
| 5 3 9 0 08-06-90 |
| 5 5 8 1 08-07-90 |
| 5 7 15 0 08-08-90 |
| 6 1 7 0 08-22-90 |
| 6 2 9 3 08-22-90 |
| 6 3 12 3 08-22-90 |
| 6 5 10 15 08-23-90 |
| 6 7 12 1 08-23-90 |
| 7 1 8 5 07-05-90 |
| 7 2 12 5 08-03-90 |
| 7 3 10 -- 11-27-89 |
| 7 5 8 17 07-06-90 |
| 7 7 7 0 12-14-89 |
| 8 1 12 9 12-12-89 |
| 8 2 9 9 07-06-90 |
| 8 3 11 27 08-09-90 |
| 8 5 10 19 01-11-90 |
| 8 7 10 -- 12-04-89 |
| 9 1 11 13 07-07-90 |
| 9 2 8 66 08-10-90 |
| 9 3 9 12 07-09-90 |
| 9 5 8 7 07-09-90 |
| 9 7 13 -- 12-05-89 |
| 10 1 12 15 08-24-90 |
| 10 2 8 0 07-09-90 |
| 10 3 13 -- 08-24-90 |
| 10 5 9 29 07-09-90 |
| 10 7 10 4 08-24-90 |
| 11 1 8 -- 11-28-89 |
| 11 2 8 33 12-11-89 |
| 11 3 11 -- 11-29-89 |
| 11 5 5 150 12-06-89 |
| 11 7 12 5 08-17-90 |
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